St. James Hospital is one of two main hospitals in Holby, the other being Holby City. In 2009, Nick Jordan went in for surgery to remove his brain tumour, after Zoe forged his signature on the consent to surgery form.

In 2017, Dylan Keogh secured Sebastian Grayling a rotation there as part of his F1 training.

Behind the scenes

St. James Hospital is frequently mentioned throughout the series, but has only been seen once on the show, in the series 24 episode "Sunset Syndrome".